Immobilization of Horseradish Peroxidase on Multi-Armed Magnetic Graphene Oxide Composite
نویسندگان
چکیده
منابع مشابه
Electrospun Chitosan-Gelatin Biopolymer Composite Nanofibers for Horseradish Peroxidase Immobilization in a Hydrogen Peroxide Biosensor
A biosensor based on chitosan-gelatin composite biopolymers nanofibers is found to be effective for the immobilization of horseradish peroxidase to detect hydrogen peroxide. The biopolymer nanofibers were fabricated by an electrospining technique. Upon optimization of synthesis parameters, biopolymers nanofibers, an average of 80 nm in diameter, were obtained and were then modified on the worki...
متن کاملStudies on Horseradish Peroxidase
The kinetics of the oxidation of p-cresol by Compound II of horseradish peroxidase has been studied by the stopped flow technique at an ionic strength of 0.11 from pH 2 to 11. In acid solution the reaction is kinetically first order in jcresol, but in the alkaline region a saturation effect attributable to complex formation is observed. At very high pH an additional second order reaction betwee...
متن کاملChemiluminescence biosensors for DNA detection using graphene oxide and a horseradish peroxidase-mimicking DNAzyme.
In this communication, we demonstrate that graphene oxide (GO) greatly inhibits the peroxidatic activity of a horseradish peroxidase-mimicking DNAzyme. Combining this observation with the unique DNA/GO interactions, an ultrasensitive GO-based chemiluminescence DNA biosensing platform is developed.
متن کاملReduced graphene oxide enhances horseradish peroxidase stability by serving as radical scavenger and redox mediator
Article history: Received 14 May 2015 Received in revised form 5 July 2015 Accepted 8 July 2015 Available online 9 July 2015 Graphene-based nanomaterials have been widely studied as high-performance matrices for enzyme immobilization and in the development of biosensors. Surface O-functionalities of graphene induce changes in chemical reactivity and electronic conductivity of nanomaterials and ...
متن کاملImmobilization of horseradish peroxidase on NH2-modified magnetic Fe3O4/SiO2 particles and its application in removal of 2,4-dichlorophenol.
Fe3O4 nanoparticles were prepared by a co-precipitation method with the assistance of ultrasound irradiation, and then coated with silica generated by hydrolysis and condensation of tetraethoxysilane. The silica-coated Fe3O4 nanoparticles were further modified with 3-aminopropyltriethoxysilane, resulting in anchoring of primary amine groups on the surface of the particles. Horseradish peroxidas...
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ژورنال
عنوان ژورنال: Food technology and biotechnology
سال: 2019
ISSN: 1334-2606,1330-9862
DOI: 10.17113/ftb.57.02.19.5832